Stem Cell Culture Supernatant

What does stem cell culture supernatant do for you?

Regain youthfulness and health through the power of stem cell culture supernatant

Stem cell culture supernatant refers to the supernatant liquid produced during the cultivation of human stem cells. This supernatant is abundant in functional ingredients, including bioactive substances like cytokines, exosomes, and various proteins secreted by stem cells. These components can be administered to repair tissues damaged by disease or to rejuvenate cells that have aged or deteriorated. The use of this supernatant is anticipated to have significant benefits for both health and beauty.

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Functional Ingredients in Culture Supernatant

Among the different types of stem cells, mesenchymal stem cells are particularly known for secreting a wealth of functional ingredients. This group includes stem cells derived from sources such as adipose tissue, deciduous dental pulp, umbilical cord, amnion, and bone marrow.The culture supernatant from these cells is rich in numerous substances, with cytokines and exosomes being especially noteworthy. These components have garnered significant attention and are the subject of extensive research.

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Functional Ingredients #1 — Cytokines

Cytokines are small proteins that transmit information to cells and produce various effects. To date, several hundred types of cytokines have been identified, with mesenchymal stem cells containing anywhere from several dozen to about two hundred types, depending on the cell type.

Cells that receive cytokines have specific structures known as receptors, which are designed to capture each cytokine. The relationship between cytokines and receptors is highly specific, similar to a key fitting into a keyhole. This specificity is consistent across all humans but does not extend across different animal species. As a result, "human-derived cytokines" are expected to have the most significant therapeutic impact on the human body.

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Functional Ingredients #2 — Exosome

Exosomes, also known as extracellular vesicles, are membrane-enclosed, sac-like structures released by cells. They carry a variety of proteins, lipids, RNA, and other molecules, and when exosomes are internalized by cells, these molecules are also taken in. It has been discovered that the content of exosomes varies depending on the type and state of the releasing cells.

Exosomes are reported to play a crucial role in transmitting information to cells through a mechanism distinct from that of cytokines. Recent findings have challenged traditional medical concepts, leading to vigorous research into the medical applications of exosomes. There is significant anticipation that they will become a key component in the future of regenerative medicine.

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Connection Between Stem Cells and Culture Supernatants

Previously, it was believed that stem cells treated diseases primarily by replacing damaged cells. However, recent research shows that the therapeutic benefits of stem cell therapy largely come from the functional ingredients secreted by mesenchymal stem cells, which repair surrounding cells. This effect, known as the paracrine effect, involves substances like cytokines and exosomes. Culture supernatant therapy isolates and utilizes this paracrine effect without using the stem cells themselves.

Since culture supernatant therapy does not contain stem cells, it is not classified as regenerative medicine under the current Japanese law (the Act on the Safety of Regenerative Medicine). Nonetheless, as a cell-free therapy, it is expected to play a significant role in the future of regenerative medicine. The composition of culture supernatant varies based on the stem cells and culture conditions used, making strict safety and environmental management essential. Our clinic collaborates with cell processing facilities to ensure consistent management of culture supernatant therapy from production to clinical application.

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Effects of stem cell culture supernatant therapy

HGF: Hepatocyte growth factor

Tissue regeneration/activity

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EGF: Epidermal growth factor

Improving dark spots and dullness, promoting cell turnover

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TGF-β: Transforming growth factor

Anti-inflammatory, wound healing

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IGF: Insulin-like growth factor

Skin regeneration, increased firmness and elasticity

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PDGF: Platelet-derived growth factor

Promotes cell division

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VEGF: Vascular endothelial growth factor

Improves firmness and elasticity, improves blood circulation, promotes hair growth

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KGF: Keratinocyte growth factor

Hair growth, hair restoration

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In addition to the above, it contains approximately 500 types of growth factors and cytokines.

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Benefits of Culture Supernatant in Stem Cell Therapy

• It has excellent proliferation and bone-building abilities, as well as the ability to suppress inflammation and enhance immune function, and to repair the nervous system (NGF).

• It fundamentally improves symptoms caused by aging, such as repairing and regenerating skin, promoting collagen production, inhibiting melanin production, regrowing hair, sagging skin, fine lines, dullness, inflammation, and thinning hair.

• It has a very high regenerative ability and can regenerate tissue, making it recommended for treating functional disorders and blood diseases such as leukemia.